Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity Convenient purification of histidine-tagged proteins, also when Ni²⁺ is not the best choice of metal ion As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. Available in the convenient and time-saving prepacked HiTrap^® and HiPrep^™ formats.

General description

IMAC Sepharose^® 6 Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.IMAC Sepharose^® 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.IMAC Sepharose^® 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose^® 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC Sepharose^® 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, IMAC Sepharose^® 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Legal Information

HiPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose productsThese products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HiTrap is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)